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Non-fasting fresh whole human peripheral blood samples, from a cohort of individuals belonging to a seven-generation pedigree including females and males aged 2968 years, were collected in the morning using EDTA-coated collection tubes (BD)

Apocynin, a NADPH oxidase (NOX) inhibitor, effectively reduced ROS and increased NO levels in endothelial (HUVEC) and hypertensive rat models, improving vascular reactivity [149]