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The A-anticalin effect is not dependent on direct interaction with A monomers or dimers As an independent test for the anti-A action of the A-anticalin, we explored its effects on neuronal hyperactivity, which was experimentally induced through the direct application of A to the hippocampus of 24 month-old wild-type mice 10,18

The membrane was blocked with 5% milk-TBST at room temperature for 1 h, primary antibodies were incubated overnight at 4 C, followed by affinipure peroxidase goat anti-rabbit or mouse IgG(H + L) secondary antibodies for 1 h at room temperature

M., Canas, P

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