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All experiments with sygl-1(ubiq) and lst-1(ubiq) were carried out at 15C to maximize tumor penetrance, except those in S4A and S4B Fig , where tumor penetrance was tested with different temperature regimens, and in S5ES5J Fig , where epistasis with fbf-1 fbf-2 was assayed at 25C

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A6 cells were added to 96 well plates at 2.5 x10 4 cells per well, incubated at 27C overnight, then exposed to 10 mM BSO or 2.5 mM cysteine for 18 hours, before determining cell viability using CyQUANT MTT assay following the manufacturers instructions