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In vitro experiments using primary hepatocytes confirm that acetate supplementation reverses these effects by activating the AMPKPPARA axis, underscoring the critical role of microbial-derived acetate in regulating hepatic energy homeostasis 169

After incubating the mixture at 25 C for 10 min, 100 l of 0.5% N-ethylmaleimide (NEM), 200 l of 0.61 M TCA, 200 l of 0.8 M orthophosphoric acid, and 200 l of 5.25% 2,2-bipyridyl (dissolved in 70% ethanol), 100 l of FeCl 3 were added

10.1111/cpr.13009 18 LongoN.FrigeniM.PasqualiM

Guide for judicious use of the paracetamol absorption technique in a study of gastric emptying rate of liquids