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Regulation by intracellular copper levels The regulation of copper homeostasis is fundamentally dependent on the copper-transporting ATPases ATP7A and ATP7B

Materials and methods Reagents and materials Oxidized glutathione (GSH) 2 , homocystine (Hcy) 2 , cystine (Cys) 2 , TCEP, benzyl bromide, 2-chloropyridine, L-lysine, N-acetylcysteine (NAC), 2-mercaptoethanesulfonic acid (MESNA), N-(2-mercaptopropionyl)-glycine (MPG), [2 S]-1-[3-mercapto-2-methylpropionyl]-L-proline (Captopril), and lipoic acid (LA), and ethylenediaminetetraacetic acid (EDTA) were received from the Sigma Aldrich Company (St

Schellhammer, 2012)